Journal: Journal of pharmaceutical analysis
Article Title: Discovering metabolic vulnerability using spatially resolved metabolomics for antitumor small molecule-drug conjugates development as a precise cancer therapy strategy.
doi: 10.1016/j.jpha.2023.02.010
Figure Lengend Snippet: Fig. 4. Enhanced tumor choline uptake through choline transporters. (A) Haematoxylin and eosin (H&E) stained image and mass spectrometry images of clinical lung cancer tissue. Histograms displayed the intensity of corresponding metabolites in paracancerous and cancerous areas (n ¼ 4 patients). The area drawn by the red and blue line refers to the cancerous area and paracancerous area, respectively. (B) H&E stained image and D9-choline ion image of lung cancer tissue at 4 h after dosing D9-choline to Lewis lung cancer (LLC) mouse. Histograms displayed the intensity of D9-choline in paracancerous and cancerous areas (n ¼ 3 mice). The area drawn by the red and blue line refers to the cancerous area and paracancerous area, respectively. (C) Intensity ratio of D9-choline of cancerous to paracancerous areas over time (n ¼ 3 mice). (D) Western blot analysis of choline transportation related protein expression in different cell lines. (E) Relative mRNA expression of choline transporter-like protein 1 (CTL1) in cancerous (n ¼ 535 patients) and paracancerous tissues (n ¼ 59 patients) of lung cancer patients. (F) Kaplan-Meier survival analysis comparing the overall survival in lung cancer patients with low CTL1 expression (n ¼ 227 patients) versus those with high CTL1 expression (n ¼ 267 patients). Gene expression and survival time data from the Cancer Genome Atlas. Mean ± standard deviation (SD), **P < 0.01, ***P < 0.001. PC: phosphatidylcholine. OCT1/2: organic cation transporter 1/2.
Article Snippet: Antibodies were purchased from Invitrogen (Thermo Fisher Scientific): choline transporter-like protein 1 (CTL1, PA5-90449), carboxylesterase (CES, PA5-109518); and Proteintech (Wuhan, China): organic cation transporter 1 (OCT1, 24617-1-AP), OCT2 (13594-1-AP).
Techniques: Staining, Mass Spectrometry, Western Blot, Expressing, Gene Expression, Standard Deviation
